A NEW product from Amsbio, CloneCatcher Gold DH5G electrocompent E coli cells, exhibit extremely high electroporation efficiencies that approach the theoretical maximum of 3.4×10^11cfu/ug pUC19 DNA. Transferring exogenous DNA into E coli is a standard laboratory method for cloning genes…
Category: nucleic acid
Digital biology gives new approach to DNA quantification
THE FIRST product based on Bio-Rad’s ‘digital biology’ technology, the QX100 droplet digital PCR system, applies sample partitioning to PCR (polymerase chain reaction) and promises a new approach to nucleic acid quantification. PCR is widely used to amplify and quantify…
Adme-Tox probes for multiplexed gene expression
PLEXPRESS says that researchers can now select from hundreds of genes and carry out reliable, accurate and cost-effective Adme-Tox (absorption, distribution, metabolism, and excretion toxicity) studies, thanks to its expanded library of pre-validated probes for multiplexed gene expression analysis. The…
Nucleic acid extraction system now CE certified
ROCHE says its newly-launched Magna Pure 96 system for automated sample preparation is designed to better fulfill the needs of diagnostic customers. This latest IVD version of Magna Pure 96 is CE-certified for use in Europe, having previously been introduced…
Imaging RNA in living cells in real time
THE IMAGING of RNA inside individual living cells seems like the stuff of science fiction, but is becoming a laboratory reality says Integrated DNA Technologies (IDT). The latest issue of Decoded, the company’s quarterly newsletter, highlights the use of IDT…
DNA sequencing kits for use with Ion Torrent
BIOO Scientific has expanded its line of kits for NGS library preparation to include the Nextflex DNA Sequencing Kit for Ion Torrent, which has been validated for use with the Ion PGM system from Life Technologies. The Nextflex DNA Sequencing…
How to go fishing in the deep end of the gene pool
AN APPLICATION note now available from Oxford Gene Technology provides scientists with valuable guidance on the use of targeted probes to improve data quality in next-generation sequencing. The note, titled Fishing for variants in the deep end of the gene…
Kits promise reduced bias through bi-directional RNA sequencing
AN EASY, flexible, and non-biased method to generate directional single, paired-end, and multiplexed libraries from mRNA- or rRNA-depleted total RNA is promised by Bioo Scientific for its NextFlex kits. Bi-directional sequencing, in both the 5′-3′ and the 3’5′ directions, means…
Delivering RNAi agents into myeloid and neuronal cells
TWO NEW proprietary transduction reagents offer the directed delivery of RNAi agents into myeloid cells and neuronal cell, both primary and in cell lines. MyeloAim and NeuroAim, available from Bioo Scientific, can transduce siRNA to targeted cells for efficient gene…
How to evaporate solvents from DNA and other specimens
THE REMOVAL of water and organic solvents from samples of DNA, RNA, and other biological specimens is a slightly more delicate task than the average laboratory evaporation procedure. Samples are typically of low volume and are heat-sensitive, and so may…