Time-lapse cell imaging in better focus

TWO new customisation tools for its IX3 inverted microscopes deliver better multi-well time-lapse images and more stable focus during long studies, says Olympus.

IX3 inverted microscope from Olympus offers easy customisation

The IX3-ZDC2 module provides focal drift compensation for accurate focus stability during long time-lapse experiments.

Updated CellSens imaging software (now version 1.14) adds an an improved Well Navigator tool for flexible multi well experimental set-up.

The company says these developments combine to create an accurate and reliable system for live cell imaging.

The ZDC2 module employs an IR laser diode to measure focus distance in order to compensate for factors such as thermal drift.

It is said to focus up to four times as fast as the previous model, and includes a fully motorised dichroic mirror movement.

This reduces acquisition times which, with continuous monitoring and an increased range of focus correction, enables fast and reliable multi well time-lapse acquisition.

The module is compatible with plastic bottom well plates, and with Olympus’s high-NA long working distance objective lenses.

Olympus says its silicone objectives provide the highest performance with spherical aberration-free imaging, giving the most insightful images with ease and efficiency.

CellSens 1.14 enables well plate scanning, with the Well Navigator making it possible to move to a specific well with a single click.

Because of the quantity of image data, CellSens 1.14 includes an improved data management system.

Image data is organised automatically according to well plate name, coordinates, or other user-defined parameters.

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