Qualified human feeder cells for iPSC reprogramming

A NEW range of human feeder cells from Amsbio are qualified for induced Pluripotent Stem Cell (iPSC) reprogramming. 

iPSCs are a type of pluripotent stem cell artificially derived from a non-pluripotent cells, typically an adult somatic cell

These newborn human foreskin fibroblasts, available under the trade name NuFF-RQ, are validated to support iPSC colony generation, providing an optimised feeder layer during mRNA reprogramming.

During cellular reprogramming, the feeder layer can greatly affect the health of the cell culture and the success of the reprogramming. While a range of inactivated fibroblasts can be used as a support layer for pluripotent cell culture, not all will support mRNA reprogramming says Amsbio.

NuFF-RQ is added to the company’s range of mouse embryonic fibroblasts (MEFs) and standard foreskin fibroblasts for experiments in stem cell culture.

Amsbio says all its feeder cells are meticulously derived and comprehensively tested to ensure robust and consistent performance, adding that fully qualified feeder cells save researchers much time and much laborious preparation work. They are also said to significantly reduce the risk of contamination in iPSC reprogramming experiments, because they have undergone comprehensive safety tests including the detection of human pathogens and mycoplasma.

NuFF-RQ qualified cells are available in conjunction with Amsbio’s highly modified synthetic 5-capped mRNAs for safe, non-integrated human cell reprogramming.

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