Confocal microscope gives enhanced imaging of living specimens

RESEARCHERS seeking more sensitive live cell imaging along with high-speed measurement capability will find the Olympus FluoView FV1200 confocal laser scanning microscope meets their needs, says the company.

Offering ultra-high sensitivity along with fluorescence measurement, this instrument is designed specifically to optimise live cell and tissue imaging. Its lower laser demands mean lower phototoxicity and photobleaching, says Olympus.

For live cell imaging, the scanhead gives increased sensitivity thanks to it silver-coated galvo mirrors, which are said to significantly increase light throughput.

The entire system is optimised for sensitivity, with increased excitation and emission transmission efficiencies, particularly in the near-infrared (IR) light range, where much of today’s key in vivo and live cell imaging research occurs.

The FV1200 also offers a 748nm diode laser for researchers doing near-IR imaging.

Further increasing sensitivity is an optional cooled gallium arsenide phosphide (GaAsP) detector unit that minimises electrical noise.

Olympus says the GaAsP detectors deliver 45 percent quantum efficiency, giving some of the highest signal-to-noise imaging ratios possible.

When the GaAsP unit is used with three conventional confocal detectors, the FV1200 can acquire up to five simultaneous fluorescent channels with its near-IR 748nm laser diode, allowing users to image DAPI, GFP, RPF, Cy5, and Cy7 simultaneously.

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