A RANGE of HPLC/UHPLC columns has been designed specifically for the analysis of proteins and peptides. The Aeris Core-Shell range is said to deliver higher resolution and peak capacities than other bioseparation media, producing sub two micron results on any LC instrument. Phenomenex says they also exhibit virtually no bleed, making them compatible with mass spectrometry.
Aeris Core-Shell columns from Phenomenex are designed specifically for protein and peptide separations
The columns are availabe in wide-pore and small-pore configurations. The wide-pore range 3.6-micron core-shell columns are optimised for separation of intact proteins and polypeptides. These are available in three selectivities – XB-C18, XB-C8, and C4. Producing lower backpressures than traditional separation media allows longer or coupled columns to be used for greater resolving power.
Aeris Peptide columns are available in 3.6-micron and 1.7-micron versions. These columns are designed with a small pore size, and are recommended for the separation of low molecular weight peptides and for peptide mapping. Peptide 3.6-micron particles offer ultra-high performance at backpressures compatible with both HPLC and UHPLC instruments. On UHPLC systems, the Phenomenex says 1.7-micron Aeris particles can deliver higher peak capacities than fully porous sub-2 micron columns.
The company says that the new Aeris columns are plug-and-play, with easy transfer of methods from other columns. “With a little method optimisation, users can achieve higher resolution and better performance, and our PhenoLogix support team is ready to help customers with their switch to Aeris”, observes Kari Kelly, Phenomenenx’s brand manager for Aeris columns.