THE BENEFITS of reversed-phase biomolecule separations can now be experienced in ultra-high-performance liquid chromatography (UHPLC), says Agilent, thanks to its new Zorbax 300SB-C3 and 300-Diphenyl chromatography columns. This range of columns has particles smaller than 2um and pores of 300 Angstrom, giving reversed-phase liquid chromatography both rapid resolution and high definition, says the company.
Zorbax UHPLC columns from Agilent promise high performance in protein separations
The full Zorbax range includes 13 phases for small molecule applications, including HILIC, and four phases for large molecule separations.
“Agilent now offers the broadest range of sub-2um, wide-pore biocolumns,” said product manager Linda Lloyd. “The new Zorbax 1.8um columns add to the scalability of the family of C18, C8 and C3 phases already available.”
Agilent says the particle and pore sizes of the new columns bring UHPLC efficiency and resolution to reversed-phase protein separations. Stable up to pressures of 1200 bar, the columns should deliver symmetrical peaks when using trifluoroacetic or formic acid mobile phase modifiers, even at low pH. Column life is said to be uncompromised by this high performance.