A NEW product from Amsbio, CloneCatcher Gold DH5G electrocompent E coli cells, exhibit extremely high electroporation efficiencies that approach the theoretical maximum of 3.4×10^11cfu/ug pUC19 DNA.
Petri dish culture of E coli
Transferring exogenous DNA into E coli is a standard laboratory method for cloning genes and constructing cDNA along with genomic and epitope libraries.
The limiting factor in many such procedures is the efficiency by which DNA can be introduced into E coli. Electroporation is one method to efficiently introduce DNA into E coli.
CloneCatcher Gold cells meet the needs of scientists seeking to find rare clones, or building complex or metagenomic libraries. It significantly outperforms all other commercially available electrocompetent cells when introducing ligated DNA, claims Amsbio, saying the product increases the chances of success when the amount of input DNA is limited.
CloneCatcher Gold requires less topoisomerase-loaded vector and can therefore can reduce overall operating costs and boost productivity as the protocol takes only ten minutes to complete.