TWO RECENTLY-presented presented research posters discussing dye and quencher phosphoramidites and CPG solid supports for oligonucleotide synthesis are available for download from Link Technologies.
The company reports that these posters generated a lot of interest when presented at the first international conference on Fluorescent Biomolecules and their Building Blocks – Design and Applications, held in Gothenburg, Sweden, earlier this month.
The first poster is titled ‘A new non-fluorescent quencher for use in oligonucleotide synthesis’, and describes the creation of a new quencher to ease the preparation of probes for use in assays where the fluorophore emits in the 550 – 600nm range.
The second, ‘Cyanine functionalised solid supports for oligonucleotide synthesis’, discusses the benefits of incorporating cyanine dyes at the 3’ end of an oligonucleotide via the use of solid CPG supports.
The greatest advantage of the method is that it eliminates the need for post-synthetic labelling onto an oligonucleotide modified with an amino linker at the 3’ end.
Dual cyanine dye labelled probes (ie cyanine 540/cyanine 650) are especially useful for detecting DNA-protein interactions using Fret.
Link Technologies’s products includes phosphoramidites and CPG solid supports for synthesising oligonucleotides. These include those labelled with fluorescent dyes such as Tamra (rhodamine), fluorescein (including 6-Fam, Hex and Tet) and Quasar dyes, as well as the dark quenchers dabcyl, Deep Dark Quencher 1 and the Black Hole Quenchers.