A RANGE of anti-tag antibodies from Amsbio, for the detection and purification of recombinant tagged proteins, are mainly mouse monoclonal antibodies which have been screened and validated applications including Western blot, immunoprecipitation, immunofluorescent staining,and flow cytometry.
The anti-DDK clone 4C5 antibody detects the DDK epitope, a widely-used tag in recombinant proteins, which is included in the pCMV6-Entry vector forming the backbone of the TrueORF cDNA series.
Linked to HRP, Dylight 488/550, Phycoerythrin and biotin, these anti-DDK provide researchers with a simpler way to detect proteins with low expression levels, and to purify recombinant proteins with high purities and yields, says Amsbio.
Anti-DDK clone 4C5 has been used to validate the expression of over 15,000 recombinant proteins from mammals, to purify more than 5000 human recombinant proteins from HEK293 cells, and to purify immunogens for the development of antibodies.